Design and Application of a New Tool for the Evaluation of the National Network of Leptospirosis Laboratories

Abstract

INTRODUCTION: Leptospirosis is an acute febrile disease, often underdiagnosed and underreported because of its diverse clinical presentation, low suspicion, and unavailability of rapid, simple and efficient diagnostic methods. The National Leptospirosis Reference Laboratory (LNRL) of the National Institute of Respiratory Diseases “Dr. Emilio Coni” produces thermo-resistant antigen (TR) and distributes it to the National Network of Leptospirosis Laboratories (RNLL). These laboratories perform the screening, send the positive samples for confirmation and the negative ones for quality control. However, until this study there was no control of TR uses and shipment of samples. OBJECTIVES: To design and implement a tool to operatively assess the RNLL. METHODS: During the years 2012 and 2013, a registration form for the TR use was applied, also creating and analyzing indicators of use efficiency, application and replacement of TR, and referral of samples. RESULTS: It was noted that in the period 2012-2013, the participation in the CC and efficiency of use of TR improved. The derivation of positive samples and request for replacement TR remained similar. CONCLUSIONS: The designed form allowed for the first time to obtain information on the quantity and quality of use of the reagent, to design and calculate indicators to assess the distribution and application of TR during 2012 and 2013. In general, the performance of decentralized laboratories improved from one year to the next.